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KMID : 0613820190290080854
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Journal of Life Science
2019 Volume.29 No. 8 p.854 ~ p.860
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A Study of the Anti-inflammatory Effect of Protein Derived from Tenebrio molitor Larvae
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Seo Min-Chul
Lee Hwa-Jeong
Lee Joon-Ha
Baek Min-Hee
Kim In-Woo
Kim Sun-Young
Hwang In-Yeong
Kim Mi-Ae
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Abstract
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This study investigated the optimum pH conditions for efficient extraction of protein from defatted Tenebrio molitor (TM) larvae. We examined the anti-inflammatory effect of protein derived from defatted TM larvae obtained by an alkaline extraction method. Six extraction pH values (7, 8, 9, 10, 11, and 12) and three precipitation pH values (2, 4, and 6) were used. The protein content, browning degree, and recovery yield of the protein obtained under each pH condition were determined. For efficient extraction of protein from defatted TM larvae, a combination of an extraction pH of 9 and precipitation pH of 4 resulted in a 32.4% recovery yield based on the extraction value and degree of browning. To determine whether the protein ameliorated inflammation by inhibition of macrophage activation by lipopolysaccharides (LPS), we measured nitric oxide (NO), cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase (iNOS) expression in LPS-stimulated raw 264.7 macrophage cells. The protein markedly inhibited the production of NO without cytotoxicity and reduced the expression level of COX-2 and iNOS protein through the regulation of mitogen-activated protein kinases (MAPKs) and nuclear factor kappa B (NF-κB) signaling. These results suggested that protein derived from TM larvae could have potential applications in anti-inflammatory therapeutic agents and protein supplements.
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KEYWORD
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Anti-inflammatory activity, mitogen-activated protein kinases, nuclear factor-kappa B, Tenebrio molitor
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